A Method for Quantitative Extraction of Urinary 5-hydroxyindoleacetic Acid.
نویسنده
چکیده
HE QUANTITATIVE ESTIMATION of urinary 5-hydroxyindoleacetic acid (5-HIAA) has attained clinical importance with increasing interest in indole metabolism. Urinary 5-HIAA levels have largely been determined by the use of an extraction procedure described by Udenfriend et al. (1). The author has found, as have others (2), that this method produces inconsistent results on varying dilutions of a given urine sample and suggests the following extraction procedure to circumvent the source of error.
منابع مشابه
Estimation of Urinary 5-hydroxyindoleacetic Acid by Paper Chromatography--a Sensitive Method.
A simple and highly sensitive method for the identification and quantitative estimation of 5-hydroxyindoleacetic acid (5-HIAA) concentration in urine is described. 5-HIAA was concentrated following extraction into ethyl acetate, and separated from other indolic acids by paper chromatography. A color reagent, p-dimethylaminocinnamaldehyde, was used to detect 5-HIAA on paper, in concentrations as...
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Udenfriend ‘s nitrosonaphthol test for 5-hydroxyindoleacetic acid (5-HIAA) is subject to interference by urinary phenolic acids and drug metabolites. Studies in our laboratory have shown that the specificity and sensitivity of the nitrosonaphthol reaction with 5-hydroxyindoles can be greatly enhanced by the addition of 2-mercaptoethanol. This observation serves as the basis of a simple, improve...
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1. Pratt, J. J., Steroid immunoassay in clinical chemistry. Clin. Chem. 24, 1869 (1978). 2. Shackleton, C. H. L., and Whitney, J. 0., Use of Sep-pak cartridges for urinary steroid extraction: Evaluation of the method for use prior to gas chromatographic analysis. Clin. Chim. Acta (in press). 3. Thenot, J.-P., and Horning, E. C., MOTMS derivatives of human steroids for GC and GC-MS studies. Anal...
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IN RECENT STUDIES (1) in which the urinary levels of 5-hydroxiiidoieacetic acid (5-HIAA) were measured by the nitrosonaphthol procedure (2), a chromophore was formed with the endogenous metabolite ill about 10% of the specimens from hospitalized patients which was not identical with that of the authentic reference compound. Although this method is widely used and is generally reiiable, the ques...
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A rapid and accurate quantitative method was developed and validated for the analysis of four urinary organic acids with nitrogen containing functional groups, formiminoglutamic acid (FIGLU), pyroglutamic acid (PYRGLU), 5-hydroxyindoleacetic acid (5-HIAA), and 2-methylhippuric acid (2-METHIP) by liquid chromatography tandem mass spectrometry (LC/MS/MS). The chromatography was developed using a ...
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عنوان ژورنال:
- Clinical chemistry
دوره 10 شماره
صفحات -
تاریخ انتشار 1964